Rapid 3’-End ssDNA Labeling Kit

Description
The Rapid 3′-End ssDNA Labeling Kit is designed to covalently attach a variety of fluorescent dyes, haptens, or clickable tags to the 3’ end of single‑stranded DNA (ssDNA). This kit works directly with ssDNA (20–120 nt) and forms a stable 3’‑terminal conjugate suitable for downstream molecular applications.
A standard reaction can label up to 4 nmol of ssDNA (20–120 nt) with a typical recovery of 80–95% after purification. Labeling efficiency may vary depending on sequence context and secondary structures at the 3’ end. The separation of labeled ssDNA from unreacted components is accomplished with a simple spin-column purification procedure, yielding clean and ready-to-use labeled products.
The resulting 3′-labeled ssDNA can be used in a broad range of applications, including:

Key Features
Direct 3’-end modification – Works with standard ssDNA (20–120 nt) without requiring special nucleotides.
High yield and recovery – Typical 80–95% recovery from 4 nmol input.
Multiplex compatible – Enables simultaneous 3’ labeling of mixed oligo pools in one reaction.
NGS-ready applications – Optimized for target enrichment, hybrid-capture, and probe-based assays.
Simple workflow – Efficient labeling and purification with a quick spin-column cleanup.

Applications
This kit enables a broad spectrum of applications in biological research, diagnostics, and analytical workflows. The 3’-end labeled single-stranded DNA (ssDNA) generated can be utilized for:
NGS target enrichment – 3′ tags enable capture, pull-down, or solid-phase immobilization of probe pools for hybrid-capture enrichment prior to library construction
Hybridization probes – Use in in situ hybridization, Southern/ Northern blotting, or microarrays where 3′-terminal tags provide detection without interfering with hybridization at the probe core.
Surface/antibody/drug conjugation – 3′ tags facilitate oriented coupling to functionalized surfaces, antibodies, or small-molecule carriers.

Storage Conditions
*Store all components at their specified temperatures, limit freeze/thaw cycles to no more than 10, protect from light exposure, and handle under aseptic conditions to maintain product quality and integrity.