Rapid 5’-End ssDNA Labeling Kit
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- Rapid 5’-End ssDNA Labeling Kit
Cat. No. | Product Name | Quantity |
P1010 | P1011 | 5’-End ssDNA Labeling Kit/with FAM | 3 Rxn | 10 Rxn |
P1020 | P1021 | 5’-End ssDNA Labeling Kit/with VIC | 3 Rxn | 10 Rxn |
P1030 | P1031 | 5’-End ssDNA Labeling Kit/with TAMRA | 3 Rxn | 10 Rxn |
P1040 | P1041 | 5’-End ssDNA Labeling Kit/with Cy3 | 3 Rxn | 10 Rxn |
P1050 | P1051 | 5’-End ssDNA Labeling Kit/with Cy5 | 3 Rxn | 10 Rxn |
P1060 | P1061 | 5’-End ssDNA Labeling Kit/with Biotin | 3 Rxn | 10 Rxn |
P1070 | P1071 | 5’-End ssDNA Labeling Kit/with DBCO | 3 Rxn | 10 Rxn |
P1080 | P1081 | 5’-End ssDNA Labeling Kit/with Azide | 3 Rxn | 10 Rxn |
Description
The Rapid 5′‑End ssDNA Labeling Kit enables precise, stable modification of single‑stranded DNA at the 5′ end using our patented conjugation technology. This targeted approach preserves probe functionality while allowing efficient attachment of fluorescent dyes, haptens, or click‑chemistry handles.
Ideal for advanced research and diagnostic applications, the kit delivers >90% labeling efficiency with 8–10 nmol yield per reaction and includes all reagents and purification components for a streamlined, high‑purity workflow.
Our kit uses a novel chemistry to form a stable 5′‑conjugate on ssDNA, ensuring long‑term label stability under stringent assay conditions. The integrated spin‑column purification allows rapid processing in just a few hours. It supports applications requiring high‑purity, high‑yield probes.
Key Features
Patented Chemistry – Stable covalent label
High Yield – Up to 8–10 nmol per reaction, >90% efficiency
Complete Kit – Includes all reagents, enzyme, catalysts, buffers, and spin‑columns
Multiple Label Options – From fluorophores to click‑chemistry handles
HPLC‑Compatible – For high-purity oligos
Fast Workflow – Label and purify in a few hours
Applications
This kit enables a broad spectrum of applications in biological research, diagnostics, and analytical workflows. The 5’-end labeled single-stranded DNA (ssDNA) generated can be utilized for:
DNA probes in hybridization techniques such as in situ hybridization, Southern blotting, and Northern blotting
Probe-based real-time PCR for sensitive and specific nucleic acid detection
Pyrosequencing and genotyping applications
Antibody-oligonucleotide or drug-oligonucleotide conjugates
DNA immobilization on beads, slides, or biosensors
Electrophoretic mobility shift assay (EMSA)
Target Gene Catcher or Enrichment for Next-Generation Sequencing (NGS), improving sensitivity while reducing sequencing costs.
Storage Conditions
*Store all components at their specified temperatures, limit freeze/thaw cycles to no more than 10, protect from light exposure, and handle under aseptic conditions to maintain product quality and integrity.
Item | Storage* |
10X Labeling Buffer | -20°C |
5′-Labeling Enzyme | -20°C |
3X Catalyst | -20°C |
Modifications | -20°C |
10X Digestion Buffer | -20°C |
Digestion Mix A | -20°C |
Digestion Mix B | -20°C |
Binding Buffer | 15-25°C |
Wash Buffer | 15-25°C |
Oligo-Purification Column | 15-25°C |
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Rapid 3’-End ssDNA Labeling Kit
Cat. No. Product Name Quantity P3010 | P3011 Dual-End ssDNA Labeling Kit/with Dye488 and BHQ1 5 Rxn | 10 Rxn P3020 | P3021 Dual-End ssDNA Labeling Kit/with VIC and BHQ1 5 Rxn | 10 Rxn P3030 | P3031 Dual-End ssDNA Labeling Kit/with TMR and BHQ2 5 Rxn | 10 Rxn P3040 | P3041 Dual-End ssDNA Labeling Kit/with CY3 and BHQ2 5 Rxn | 10 Rxn P3050 | P3051 Dual-End ssDNA Labeling Kit/with CY5 and BHQ2 5 Rxn | 10 Rxn DescriptionThe Rapid 3′-End ssDNA Labeling Kit is designed to covalently attach a variety of fluorescent dyes, haptens, or clickable tags to the 3’ end of single‑stranded DNA (ssDNA). This kit works directly with ssDNA (20–120 nt) and forms a stable 3’‑terminal conjugate suitable for downstream molecular applications.A standard reaction can label up to 4 nmol of ssDNA (20–120 nt) with a typical recovery of 80–95% after purification. Labeling efficiency may vary depending on sequence context and secondary structures at the 3’ end. The separation of labeled ssDNA from unreacted components is accomplished with a simple spin-column purification procedure, yielding clean and ready-to-use labeled products.The resulting 3′-labeled ssDNA can be used in a broad range of applications, including: Key FeaturesDirect 3’-end modification – Works with standard ssDNA (20–120 nt) without requiring special nucleotides.High yield and recovery – Typical 80–95% recovery from 4 nmol input.Multiplex compatible – Enables simultaneous 3’ labeling of mixed oligo pools in one reaction.NGS-ready applications – Optimized for target enrichment, hybrid-capture, and probe-based assays.Simple workflow – Efficient labeling and purification with a quick spin-column cleanup. ApplicationsThis kit enables a broad spectrum of applications in biological research, diagnostics, and analytical workflows. The 3’-end labeled single-stranded DNA (ssDNA) generated can be utilized for:NGS target enrichment – 3′ tags enable capture, pull-down, or solid-phase immobilization of probe pools for hybrid-capture enrichment prior to library constructionHybridization probes – Use in in situ hybridization, Southern/ Northern blotting, or microarrays where 3′-terminal tags provide detection without interfering with hybridization at the probe core.Surface/antibody/drug conjugation – 3′ tags facilitate oriented coupling to functionalized surfaces, antibodies, or small-molecule carriers. Storage Conditions*Store all components at their specified temperatures, limit freeze/thaw cycles to no more than 10, protect from light exposure, and handle under aseptic conditions to maintain product quality and integrity. Item Storage* 10X 3′-Labeling Buffer -20°C 10X Catalyst A -20°C 40X Catalyst B -20°C 50X Catalyst C -20°C Biotin (50 mM) -20°C 3’-Labeling Mix A (350 U/μL) -20°C 3’-Labeling Mix B (40 U/μL) -20°C Binding Buffer 15-25°C Wash Buffer 15-25°C Oligo-Purification Column 15-25°C
Rapid Dual-End ssDNA Labeling Kit
Cat. No. Product Name Quantity P3010 | P3011 Dual-End ssDNA Labeling Kit/with Dye488 and BHQ1 5 Rxn | 10 Rxn P3020 |P3021 Dual-End ssDNA Labeling Kit/with VIC and BHQ1 5 Rxn | 10 Rxn P3030 | P3031 Dual-End ssDNA Labeling Kit/with TMR and BHQ2 5 Rxn | 10 Rxn P3040 | P3041 Dual-End ssDNA Labeling Kit/with CY3 and BHQ2 5 Rxn | 10 Rxn P3050 | P3051 Dual-End ssDNA Labeling Kit/with CY5 and BHQ2 5 Rxn | 10 Rxn DescriptionThe Rapid Dual-End ssDNA Labeling Kit enables covalent modification of both 5′ and 3′ ends of ssDNA using our patented conjugation technology. This chemistry supports sequential attachment of two distinct labels, enhancing probe versatility for multiplexed detection and diagnostic applications.Optimized for 20–30 nt ssDNA, the kit achieves ~50% dual-labeling efficiency with 1 nmol yield per reaction. It includes all reagents and spin-column purification for a fast, high-purity workflow.Available dye–quencher combinations include Dye488/BHQ1, VIC/BHQ1, TMR/BHQ2, Cy3/BHQ2, and Cy5/BHQ2, covering a wide fluorescence detection range. Key FeaturesDual 5’/3’ labeling – Enables covalent modification at both termini of ssDNA for versatile probe design.Patented chemistry – Provides stable dual-end linkage through proprietary conjugation.Optimized for short oligos – Designed for 20–30 nt ssDNA with ~50% labeling efficiency.Balanced yield – Produces up to 1 nmol of purified dual-labeled probes per reaction.Streamlined workflow – All-in-one reagents with rapid spin-column purification.Flexible dye options – Available in five dye–quencher pairs: Dye488/BHQ1, VIC/BHQ1, TMR/BHQ2, Cy3/BHQ2, and Cy5/BHQ2. ApplicationsMultiplex hybridization / assays – Dual-end labeling enables simultaneous multi-target detection and signal normalization, enhancing assay throughput and accuracy.FRET / TaqMan qPCR/dPCR – Fluorophore–quencher (or donor–acceptor) pairing at both ends allows real-time monitoring of target binding or conformational changes for precise quantitative PCR.Hybrid capture / NGS – One terminus supports solid-phase capture while the other provides detection or barcoding, improving target enrichment and sequencing efficiency.Clinical quantification – Suitable for viral load monitoring, gene expression, and CNV analysis, delivering stable and highly sensitive quantification.
